Lactate Dehydrogenase A Antikörper (Biotin)

Produktnummer ABIN568298

Der Schaf Polyklonal Anti-Lactate Dehydrogenase A-Antikörper wurde für WB, IP, IF, EIA, DB, ID und RIA validiert. Er ist geeignet, Lactate Dehydrogenase A in Proben von Kaninchen zu detektieren.

Kurzübersicht für Lactate Dehydrogenase A Antikörper (Biotin) (ABIN568298)

Target: Lactate Dehydrogenase A (LDHA)

Reaktivität: Kaninchen

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Lactate Dehydrogenase A Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)

Produktdetails anti-Lactate Dehydrogenase A Antikörper (Biotin)

Spezifität: L-Lactic Dehydrogenase from Rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: Molar Ratio: Biotin/IgG ~ 6.6

Aufreinigung: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: L-Lactic Dehydrogenase is isolated and purified from Rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore by adding 1.0 mL of sterile distilled water

Konzentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Details zu Lactate Dehydrogenase A

Target: Lactate Dehydrogenase A (LDHA)

Andere Bezeichnung: LDHA

Hintergrund: Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).Synonyms: Cell proliferation-inducing gene 19 protein, L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-A, LDH-M, NY-REN-59, PIG19, Renal carcinoma antigen NY-REN-59

Gen-ID: 100009107

NCBI Accession: NP_001075746

UniProt: P13491

Pathways: Warburg Effekt