Hexokinase 1 Antikörper (Biotin)

Produktnummer ABIN568253

Der Kaninchen Polyklonal Anti-Hexokinase 1-Antikörper wurde für WB, IP, IF, EIA, RIA, ID und DB validiert. Er ist geeignet, Hexokinase 1 in Proben von Saccharomyces cerevisiae zu detektieren.

Kurzübersicht für Hexokinase 1 Antikörper (Biotin) (ABIN568253)

Target: Hexokinase 1 (HK1)

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Hexokinase 1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Immunodiffusion (ID), Dot Blot (DB)

Produktdetails anti-Hexokinase 1 Antikörper (Biotin)

Spezifität: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.

Produktmerkmale: Molar Ratio: Biotin/IgG = ~5.4

Aufreinigung: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

Immunogen: Hexokinase isolated and purified from Baker’s Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Details zu Hexokinase 1

Target: Hexokinase 1 (HK1)

Andere Bezeichnung: Hexokinase-1

Hintergrund: Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia. In yeast there are three glucose-phosphorylating isoenzymes, designated hexokinase I, II and glucokinase. Present with 40800 Molecules/cell in log phase SD medium.Synonyms: HKA, HXK1, Hexokinase PI, Hexokinase-A

Gen-ID: 850614

NCBI Accession: NP_116711

UniProt: P04806

Pathways: Carbohydrate Homeostasis, Warburg Effekt