Ceruloplasmin Antikörper

Produktnummer ABIN568138

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Ceruloplasmin in WB, IP, IHC (p), IF, EIA, RIA, ID und DB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Ceruloplasmin Antikörper (ABIN568138)

Target: Ceruloplasmin (CP) (Ceruloplasmin (Ferroxidase) (CP))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ceruloplasmin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Immunodiffusion (ID), Dot Blot (DB)

Produktdetails anti-Ceruloplasmin Antikörper

Spezifität: The antibody recognizes Ceruloplasmin from Human serum. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), Block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Aufreinigung: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Ceruloplasmin isolated and purified from Human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore by adding 1.0 mL of sterile distilled water. Prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2) and preferably use the same day.

Konzentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Ceruloplasmin

Target: Ceruloplasmin (CP) (Ceruloplasmin (Ferroxidase) (CP))

Andere Bezeichnung: Ceruloplasmin

Hintergrund: Caeruloplasmin is a copper binding plasma glycoprotein consisting of a single polypeptide chain. It has a molecular weight of 132 kDa and occurs in plasma at a concentration of 150-500 mg/L. Although the physiological functions of Caeruloplasmin are not fully understood it is implicated in a number of possible functions. 95 % of plasma copper is bound to Caeruloplasmin, implying some involvement in copper transport. Caeruloplasmin has been described as both an oxidant and an antioxidant, its exact role or roles in this respect are not clear. Plasma levels of Caeruloplasmin are elevated after inflammation and trauma and it is therefore classified as an acute phase protein. The measurement of Caeruloplasmin in plasma is useful in the diagnosis of Wilson's disease, where levels are greatly reduced. Caeruloplasmin is extremely labile in plasma and rapidly degrades to 115 kDa and 19 kDa fragments. The age of plasma samples should therefore be taken into account when interpreting immunological analysis of caeruloplasmin.Synonyms: CP, Ferroxidase

Gen-ID: 1356

NCBI Accession: NP_000087

UniProt: P00450

Pathways: Transition Metal Ion Homeostasis