Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

DYKDDDDK Tag Antikörper

Der Maus Monoklonal Anti-DYKDDDDK Tag-Antikörper wurde für WB, ELISA, IP, DB und ISt validiert und wurde unabhängig validiert. Er ist geeignet, DYKDDDDK Tag in Proben von zu detektieren.
Produktnummer ABIN559688

Kurzübersicht für DYKDDDDK Tag Antikörper (ABIN559688)

Target

DYKDDDDK Tag

Reaktivität

Bitte anfragen

Wirt

  • 65
  • 54
  • 10
  • 8
  • 6
Maus

Klonalität

  • 76
  • 66
Monoklonal

Konjugat

  • 50
  • 15
  • 10
  • 6
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser DYKDDDDK Tag Antikörper ist unkonjugiert

Applikation

  • 87
  • 61
  • 34
  • 27
  • 25
  • 22
  • 19
  • 13
  • 12
  • 10
  • 6
  • 6
  • 5
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Immunostaining (ISt)

Klon

FG4R
  • Sequenz

    DYKDDDDK

    Spezifität

    Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins

    Sensitivität

    WB (with ECL): 1-5ng of DYKDDDDK-tagged fusion proteins

    Aufreinigung

    Protein A affinity chromatography from mouse ascites fluid

    Immunogen

    DYKDDDDK (DYKDDDDK) synthetic peptide conjugated to KLH

    Isotyp

    IgG1
  • Applikationshinweise

    WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Validierung #102796 (Immunofluorescence)
    'Independent Validation' Siegel
    by
    Martinelli lab, Neuroscience Department, UConn Health
    No.
    #102796
    Datum
    13.02.2018
    Antigen
    DYKDDDDK-tag
    Chargennummer
    02017
    Validierte Anwendung
    Immunofluorescence
    Positivkontrolle
    African green monkey kidney fibroblast-like COS7 cells transfected with BAI3, a transmembrane protein with a surface presented DYKDDDDK-tag
    Negativkontrolle
    untransfected COS7 cells
    Bewertung

    Passed. Signal was observed as expected. There was never any signal on untransfected cells, demonstrating specificity of the antibody.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN1715174
    Sekundärantikörper
    goat anti-mouse AF488 conjugated antibody (Life Technologies, A11029, lot 1829920)
    Full Protocol
    • Grow COS7 cells in DMEM (Biological Industries, 01-052-1A, lot 1728619) supplemented with FBS (Hyclone, SH3039602) without antibiotics, at 37°C and 5% CO2 in 1ml on glass coverslips on 24-well plate.
    • Transfect cells with calcium phosphate method with a eukaryotic expression construct to express a DYKDDDDK-tagged BAI3, which will be surface presented.
    • Grow cells for 24h.
    • Fix cells on coverslips in 4% PFA for 15min at 4°C.
    • Wash cells 3x for 5min with PBS.
    • Block non-specific binding with 5% goat serum in PBS for 1h at RT.
    • Incubate cells with primary mouse anti-DYKDDDDK-tag antibody (antibodies-online, ABIN1715174, lot 02017) diluted 1:2000 in blocking solution ON at 4°C.
    • Wash cells 3x for 5min with PBS.
    • Incubate cells with secondary goat anti-mouse AF488 conjugated antibody (Life Technologies, A11029, lot 1829920) diluted 1:400 in blocking buffer for 1h at RT protected from light.
    • Wash cells 3x for 5min with PBS.
    • Mount coverslips on glass slides in Fluoromount-G with DAPI mounting medium (ThermoFisher Scientific, 00-4959-52, lot B2215-N915).
    • Image acquisition on a Zeiss Axiovert epifluorescence with with a 40x objective, using a Zeiss ApoTome for optical sectioning.
    Anmerkungen
  • Konzentration

    1mg/mL

    Buffer

    10mM PBS, pH 7.2, 0.05% NaN3

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C
  • Target

    DYKDDDDK Tag

    Andere Bezeichnung

    FLAG Tag

    Substanzklasse

    Tag
Sie sind hier:
Chat with us!