COL1A1 Antikörper (Biotin)

Produktnummer ABIN5596821

Hersteller Produkt- Nr.: 600-406-103

Der Kaninchen Polyklonal Anti-COL1A1-Antikörper wurde für WB, IHC, ELISA, IP, FACS, DB und MA validiert. Er ist geeignet, COL1A1 in Proben von Human, Ratte und Maus zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für COL1A1 Antikörper (Biotin) (ABIN5596821)

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL1A1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Dot Blot (DB), Multiplex Assay (MA)

Produktdetails anti-COL1A1 Antikörper (Biotin)

Hersteller Produkt- Nr.: 600-406-103

Hersteller: Rockland

Verwendungszweck: Collagen Type I Antibody Biotin Conjugated

Kreuzreaktivität (Details): Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Produktmerkmale: Synonyms: rabbit anti-collagen type I antibody biotin conjugation, biotin conjugated rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1

Aufreinigung: This product has been prepared by immunoaffinity chromatography using immobilized antigens.

Immunogen:

Immunogen: Collagen Type I from human and bovine placenta.

Immunogen Type: Native Protein

Isotyp: IgG

Labeling Ratio: 10-20

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-COLLAGEN Type I Antibody Biotin Conjugated has been tested by dot blot and Flow Cytometry and is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:3,000 - 1:6,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:3,000 - 1:6,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-COL1A1 Antikörper (Biotin) (ABIN5596821)

Heukels, van Hulst, van Nimwegen, Boorsma, Melgert, van den Toorn, Boomars, Wijsenbeek, Hoogsteden, von der Thüsen, Hendriks, Kool, van den Blink: "Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis." in: Respiratory research, Vol. 19, Issue 1, pp. 90, (2018) (PubMed).

Sinha, Hoshino, Hong, Kirkbride, Grega-Larson, Seiki, Tyska, Weaver: "Cortactin promotes exosome secretion by controlling branched actin dynamics." in: The Journal of cell biology, Vol. 214, Issue 2, pp. 197-213, (2017) (PubMed).

Collins, Chan-Li, Oh, Vigeland, Limjunyawong, Mitzner, Powell, Horton: "Vaccinia vaccine-based immunotherapy arrests and reverses established pulmonary fibrosis." in: JCI insight, Vol. 1, Issue 4, pp. e83116, (2016) (PubMed).

Zhang, Maric, DiPrima, Khan, Orentas, Kaplan, Mackall: "Fibrocytes represent a novel MDSC subset circulating in patients with metastatic cancer." in: Blood, Vol. 122, Issue 7, pp. 1105-13, (2013) (PubMed).

Details zu COL1A1

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Andere Bezeichnung: COL1A1

Hintergrund: Background: COLLAGEN Type I Antibody Biotin Conjugated is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.

Gen-ID: 1277

NCBI Accession: NP_000079

UniProt: P02452

Pathways: Sensory Perception of Sound, Autophagie, Growth Factor Binding