alpha 2 Macroglobulin Antikörper (HRP)

Produktnummer ABIN5596814

Hersteller Produkt- Nr.: 200-103-207-0100

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch alpha 2 Macroglobulin in WB, IHC, ELISA und DB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für alpha 2 Macroglobulin Antikörper (HRP) (ABIN5596814)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser alpha 2 Macroglobulin Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Dot Blot (DB)

Produktdetails anti-alpha 2 Macroglobulin Antikörper (HRP)

Hersteller Produkt- Nr.: 200-103-207-0100

Hersteller: Rockland

Verwendungszweck: Alpha-2-Macroglobulin Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified a2-Macroglobulin [Human Plasma].

Produktmerkmale: Synonyms: goat anti-Alpha-2-Macroglobulin Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Alpha-2-Macroglobulin Antibody, A2M antibody, Alpha 2 M antibody, Alpha 2M antibody, CPAMD5 antibody, DKFZp779B086 antibody, FWP007 antibody, S863 7 antibody

Aufreinigung: Anti-Alpha 2-Macroglobulin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: a2-Macroglobulin [Human Plasma]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:50 - 1:250

Application Note: Anti-Alpha 2-Macroglobulin has been tested by dot blot and western blot and is suitable to be assayed against 1.0 μg of alpha-Macroglobulin [Human Plasma] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:100 - 1:500

ELISA Dilution: 1:400 - 1:2,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Andere Bezeichnung: A2M

Hintergrund: Background: Alpha 2-Macroglobulin is the largest major nonimmunoglobulin protein in plasma. The alpha 2-macroglobulin molecule is synthesized mainly in liver, but also locally by macrophages, fibroblasts, and adrenocortical cells. Alpha 2 macroglobulin acts as an antiprotease and is able to inactivate an enormous variety of proteinases. It functions as an inhibitor of fibrinolysis by inhibiting plasmin and kallikrein. It functions as an inhibitor of coagulation by inhibiting thrombin. Alpha 2-macroglobulin may act as a carrier protein because it also binds to numerous growth factors and cytokines, such as platelet-derived growth factor, basic fibroblast growth factor, TGF-β, insulin, and IL-1β. Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Gen-ID: 2

NCBI Accession: NP_000005

UniProt: P01023

Pathways: Lipid Metabolism