Fibronectin Antikörper (Biotin)

Details zu Produkt Nr. ABIN5596762
Antigen
Reaktivität
Rind (Kuh), Human, Affe, Maus, Ratte (Rattus)
316
113
105
45
19
14
4
3
3
2
2
1
1
Wirt
Kaninchen
217
160
8
5
2
Klonalität
Polyklonal
Konjugat
Biotin
31
16
12
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
164
145
108
107
81
67
50
46
34
32
27
26
26
14
13
7
5
2
2
2
1
1
1
1
1
1
Optionen
Immunogen

Immunogen: Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE.

Immunogen Type: Native Protein

Isotyp IgG
Kreuzreaktivität (Details) Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.
Reinigung Anti-fibronectin (rabbit) antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificity.
Labeling Ratio 10-20
Antigen
Hintergrund

Synonyms: CIG antibody, Cold insoluble globulin antibody, LETS antibody, Migration stimulating factor antibody, MSF antibody, Transformation sensitive protein. Antibody

Background: Fibronectin antibody reacts with human fibronectin in liver, tonsil, skin and kidney. Traces of contaminating antibodies have been removed by solid-phase absorption. Biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin, an attraction that is the strongest and most stable non-covalent interaction known. Fibronectin is found in two forms in vertebrates: soluble and insoluble. Soluble plasma fibronectin is contained in blood plasma and constitutes a large protein component. Insoluble cellular fibronectin is a large component of the extra-cellular matrix where it is secreted by many different types of cells. Fibronectin plays a large role in wound healing and cell development. Anti-fibronectin (rabbit) antibody is ideal for investigators in Cardiology, Cell Biology, Microbiology, and Immunology research.

Gene Name: FN1

Gen-ID 2335
UniProt P02751
Applikations-hinweise

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-fibronectin (rabbit) antibody was assayed by immunoblot and found to be reactive against Fibronectin at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 μg of Fibronectin in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Haltbarkeit 12 months
Bilder des Herstellers
Image no. 1 for anti-Fibronectin antibody (Biotin) (ABIN5596762) Immunohistochemistry of Rabbit Anti-Fibronectin Antibody. Tissue: human kidney at pH6...
Image no. 2 for anti-Fibronectin antibody (Biotin) (ABIN5596762) Immunohistochemistry with rabbit anti fibronectin biotin conjugated at 20X with negat...
Image no. 3 for anti-Fibronectin antibody (Biotin) (ABIN5596762) Immunohistochemistry of Rabbit Anti-Fibronectin Antibody. Tissue: human kidney at pH9...
'Independent Validation' Siegel
Antigen Fibronectin
Chargennummer 39102
Validierte Anwendung Unfolding Profile
Positivkontrolle ABIN5596762
Bewertung

Passed. ABIN5596762 showed Ti at 63.4°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

Protokoll
  • Dilute ABIN5596762 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 30µl at a concentration of 0.5µM.
  • Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
  • Run Tycho measurement.
Anmerkungen

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Validierungsbilder
Unfolding Profile validation image for anti-Fibronectin antibody (Biotin) (ABIN5596762) Unfolding profile of ABIN5596762. The fluorescence signal is plotted against temperat...