Rekombinanter EED Antikörper (Sortase,His tag,AVI tag)

Produktnummer ABIN5563967

Dieser Maus Monoklonal Antikörper detektiert spezifisch EED in ELISA, ChIP-seq und ChIP. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter EED Antikörper (Sortase,His tag,AVI tag) (ABIN5563967)

Target: EED (Embryonic Ectoderm Development (EED))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EED Antikörper ist konjugiert mit Sortase,His tag,AVI tag

Applikation: ELISA, ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-EED Antikörper (Sortase,His tag,AVI tag)

Verwendungszweck: AbFlex EED antibody (rAb)

Marke: AbFlex®

Produktmerkmale: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

Aufreinigung: Ni-NTA

Immunogen: This EED antibody was raised against full-length recombinant human EED protein.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ChIP-Seq: 4 μg per ChIP Bead-based ELISA: 4 - 26 ng/mL AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61203.

Kommentare:

This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu EED

Target: EED (Embryonic Ectoderm Development (EED))

Andere Bezeichnung: EED

Hintergrund: AbFlex EED antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. EED (extraembryonic ectoderm) is a component of the Polycomb group (PcG) multiprotein complex PRC2. The PRC2 complex also contains EZH2 and SUZ12 and is recruited to Polycomb response elements and results in the methylation of histone H3 and lysine 27 and the silencing of gene expression. PRC2 initiates repression of the Hox genes during development and is involved in the maintenance of stem cell pluripotency. EED is a member of the superfamily of WD-40 repeat protein family and is required for PRC2-mediated H3K27 methylation. The PRC2 complex may also recruit platform DNMTs, thereby linking two systems of epigenetic repression.

Molekulargewicht: 57 kDa

NCBI Accession: NP_003788