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ARAF Antikörper

Name: ARAF Antikörper
SKU: ABIN5557283
Price: 456.42 EUR
Availability: InStock

Dieses Anti-ARAF-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von ARAF in WB, IF (cc) und IHC (p). Geeignet für Human und Maus.

HeLa cell lysates probed with A-RAF (24D1) Monoclonal Antibody at 1:1000 overnight at 4˚C. (ARAF Antikörper)

Paraformaldehyde-fixed, paraffin embedded human kidney section, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer at 37°C for 20min, Antibody incubation with A-RAF (24D1) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining. (ARAF Antikörper)

IHC (p)

NIH/3T3 cells were stained with A-RAF (24D1) Monoclonal Antibody at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection. (ARAF Antikörper)

IF (cc)

HeLa cells were stained with A-RAF (24D1) Monoclonal Antibody at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection. (ARAF Antikörper)

IF (cc)

4 Abbildungen

Produktnummer ABIN5557283

Kurzübersicht für ARAF Antikörper (ABIN5557283)

Target

Alle ARAF Antikörper anzeigen

ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reaktivität

Human, Maus

Wirt

Kaninchen

Klonalität

Monoklonal

Konjugat

Dieser ARAF Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon

24D1

Discover our top product ARAF Primärantikörper

Kreuzreaktivität

Human, Maus

Aufreinigung

Purified by Protein A.

Immunogen

Recombinant human A-RAF protein, around 150-300aa.

Isotyp

IgG
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 151-250) antibody
ARAF Reaktivität: Human WB, ELISA, PLA Wirt: Maus Monoclonal 3E2 unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 1-606) antibody
ARAF Reaktivität: Human WB, IF, PLA Wirt: Kaninchen Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (C-Term) antibody
ARAF Reaktivität: Human, Maus WB, IF, IHC (p), ICC Wirt: Kaninchen Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 183-199) antibody
ARAF Reaktivität: Human, Maus, Ratte, Hund, Huhn WB, IHC, IF, IP Wirt: Maus Monoclonal 1-A unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 160-310) antibody
ARAF Reaktivität: Human ELISA, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (pTyr301), (pTyr302) antibody
ARAF Reaktivität: Human, Maus WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (pSer299) antibody
ARAF Reaktivität: Human WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (Internal Region) antibody
ARAF Reaktivität: Human, Maus, Ratte WB, ELISA, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 276-325), (pTyr302) antibody
ARAF Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (Internal Region) antibody
ARAF Reaktivität: Human, Maus, Ratte, Affe WB, ELISA Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Target

ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Andere Bezeichnung

A-RAF

Hintergrund

Synonyms: Serine/threonine-protein kinase A-Raf, ARAF, Proto-oncogene A-Raf, Proto-oncogene A-Raf-1, Proto-oncogene Pks, ARAF1, PKS, PKS2
Background: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.

Gen-ID

369

UniProt

P10398

Pathways

MAPK Signalweg, RTK Signalweg, Hepatitis C