Topoisomerase II alpha Antikörper (C-Term)

Produktnummer ABIN5542013

Dieser Maus Monoklonal Antikörper detektiert spezifisch Topoisomerase II alpha in WB, IF, IHC (p), FACS, IP und ICC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Topoisomerase II alpha Antikörper (C-Term) (ABIN5542013)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: 1C5

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody detects 170 kDa of human Topo II alpha on Western blot with total cell lysate from human cell lines.

Kreuzreaktivität (Details): Does not rect with Mouse (WR19L, NIH/3T3), Rat (PC12, Rat-1) or Hamster (BHK) on Western blot.

Aufreinigung: Protein A agarose

Immunogen: Recombinant human Topoisomerase II alpha protein corresponding to C-termional 182 aa

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blot: 0.1-0.5 μg/mL for chemiluminescence detection system. Immunoprecipitation: 1-10 μg/200-300 μg/mL of cell extract. Immunohoistochemistry on paraffin sections: Heat treatment is necessary Microwave oven 2 times for 10 minutes aech in 10 mM citrate buffer ( pH 6.5). Immunocytochemistry: 1-10 μg/mL. Flow cytometry: 0.1-1 μg/mL (final contentration).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Konservierungsmittel: Azide free

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: top2a

Hintergrund: < div dir=ltr data-font-name=g_font_p0_4 data-canvas-width=519.3466821444035> Topoisomerase II alpha plays important roles in synthesis and transcription of DNA as well < span style=font-size: 12px> as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- and M-phases in transformed and developmentally regulated normal cells. Topoisomerase II alpha is also implicated in drug resistance of tumor cells.

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases