Topoisomerase II alpha Antikörper (C-Term)

Produktnummer ABIN5542013

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Topoisomerase II alpha in WB, IF, FACS, IHC (p), IP und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Topoisomerase II alpha Antikörper (C-Term) (ABIN5542013)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: 1C5

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody detects 170 kDa of human Topo II alpha on Western blot with total cell lysate from human cell lines.

Kreuzreaktivität (Details): Does not rect with Mouse (WR19L, NIH/3T3), Rat (PC12, Rat-1) or Hamster (BHK) on Western blot.

Aufreinigung: Protein A agarose

Immunogen: Recombinant human Topoisomerase II alpha protein corresponding to C-termional 182 aa

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blot: 0.1-0.5 μg/mL for chemiluminescence detection system. Immunoprecipitation: 1-10 μg/200-300 μg/mL of cell extract. Immunohoistochemistry on paraffin sections: Heat treatment is necessary Microwave oven 2 times for 10 minutes aech in 10 mM citrate buffer ( pH 6.5). Immunocytochemistry: 1-10 μg/mL. Flow cytometry: 0.1-1 μg/mL (final contentration).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Konservierungsmittel: Azide free

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: top2a

Hintergrund: < div dir=ltr data-font-name=g_font_p0_4 data-canvas-width=519.3466821444035> Topoisomerase II alpha plays important roles in synthesis and transcription of DNA as well < span style=font-size: 12px> as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- and M-phases in transformed and developmentally regulated normal cells. Topoisomerase II alpha is also implicated in drug resistance of tumor cells.

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases