PODXL Antikörper

Produktnummer ABIN5541389

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PODXL in FACS und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PODXL Antikörper (ABIN5541389)

Target: PODXL (Podocalyxin-Like (PODXL))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PODXL Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 53D11

Produktdetails anti-PODXL Antikörper

Spezifität: This antibody reacts with human Podocalyxin/PCLP1.

Aufreinigung: Protein A agarose

Immunogen: CHO cell expressing full length human Podocalyxin/PCLP1

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry : 10-20 μg/mL (final concentration). For details see protocol below. I mmunohistochemistry : Clone 53D11 has been reported by customer to stain Podocalyxin in Formalin-Fixed, Paraffin-Embedded human kidney tissue at an antibody concentration of 10 μg/mL.

Protokoll: Flow cytometric analysis for floating cells We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2 % fetal calf serum (FCS) and 0.1 % NaN 3]. 2) Resuspend the cells with washing buffer (5x10e6 cells/mL). 3) Add 50 μ L of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration. 4) Add 10 μ L of Clear Back (human Fc receptor blocking reagent) and 0.1 % NaN 3 to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 5) Add 30 μL of the Anti-Human Podocalyxin/PCLP1 monoclonal antibody (53D11) (10-20 μ g/mL) diluted with the washing buffer. Mix well and incubate for 30 minutes at room temperature. 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration. 7) Add 30 μL of secondary antibody (1:40 FITC conjugated anti-mouse IgG) diluted with the washing buffer. Mix well and incubate for 15 minutes at room temperature. 8) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration. 9) Resuspend the cells with 500 μ L of the washing buffer and analyze by a flow cytometer. (Positive control for flow cytometry transfectant)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Konservierungsmittel: Azide free

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Details zu PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Andere Bezeichnung: podocalyxin,podxl

Hintergrund: Recent studies with avian embryos and murine embryonic stem cells have suggested that hematopoietic cells are derived from hemangioblasts, the common precursors of hematopoietic and endothelial cells. Hara et al. molecularly clon ed podocalyxin-like protein 1 (PCLP1) as a novel surface marker for endothelial-like cells in the AGM (aorta-gonad-mesonephros) region of mouse embryos, where long-term repopulating hematopoietic stem cells (LTR-HSCs) are known to arise. PCLP1 + CD45 - cells in the AGM region incorporated acetylated low-density lipopro tein and produced both hematopoietic and endothelial cells when cocultured with OP9 stromal cells. Moreover, multiple lineages of hematopoietic cells were generated in vivo when PCLP1 + CD45 - cells were injected into neonatal liver of busulfan-treated mice. Today it is reported that the PCLP1 is identical with the Podocalyxin.

UniProt: O00592

Pathways: Tube Formation