Sonic Hedgehog Antikörper (N-Term)

Produktnummer ABIN5540856

Der Ratte Monoklonal Anti-Sonic Hedgehog-Antikörper wurde für WB, IHC (fro) und Neut validiert. Er ist geeignet, Sonic Hedgehog in Proben von Maus zu detektieren.

Kurzübersicht für Sonic Hedgehog Antikörper (N-Term) (ABIN5540856)

Target: Sonic Hedgehog (SHH)

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Sonic Hedgehog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Neutralization (Neut)

Klon: 6K12

Produktdetails anti-Sonic Hedgehog Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody detects Mouse Shh with Western Blot. Species: Mouse.

Aufreinigung: Affinity Chromatography on Protein A/G

Immunogen: Recombinant Mouse Sonic Hedgehog (Shh) N-Terminal fragment.

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/250-1/1000. Neutralization. Immunohistochemistry on Frozen Sections: 1/50-1/200.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purification: Affinity Chromatography on Protein A/G

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Hintergrund: Human Shh cDNA encodes a 462 amino acid (aa) residue (45 kDa) precursor protein with a 23 aa signal peptide. An autocatalytic cleavage reaction yields a 19 kDa (residues 24 - 197) amino-terminal fragment (Shh-N), and a 25 kDa (residues 198 - 462) carboxy-terminal domain (Shh-C). The N-terminal domain retains all known signaling capabilities, while the C-terminal domain is responsible for the intramolecular processing, acting as a cholesterol transferase that covalently transfers the cholesterol molecule to the C-terminus of Shh-N. When Shh is expressed in insect or mammalian cells, a palmitoyl group is also attached to the N-terminal cysteine of Shh-N via an amide linkage. Although the binding affinity to their receptors is not changed, lipid-modified Shh-N proteins are more potent than the unmodified proteins in cell-based assays. Other hydrophobic modifications to unmodified Shh-N, including the substitution of the N-terminal cysteine residue with two hydrophobic isoleucine residues, can also increase Shh-N potency.

UniProt: Q62226

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development