PRAME Antikörper (C-Term)

Produktnummer ABIN5540467

Dieses Anti-PRAME-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PRAME in WB und ELISA (Detection). Geeignet für Human.

Kurzübersicht für PRAME Antikörper (C-Term) (ABIN5540467)

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRAME Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA (Detection)

Produktdetails anti-PRAME Antikörper

Bindungsspezifität: AA 321-509, C-Term

Spezifität: This antibody detects PRAME / MAPE at C-term.

Aufreinigung: Protein A chromatography

Immunogen: Highly pure (> 95%) recombinant human PAME /MAPE C-terminal end (Met321-Asn509) derived from E. coli

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 1-5 μg/mL. ELISA: 0.1-1 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store lyophilized at 2-8°C for 6 month or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu PRAME

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Andere Bezeichnung: prame,mape

Hintergrund: PRAME/MAPE/OIP4 is a germinal tissue-specific gene that is also expressed at high levels in haematological malignancies and solid tumors. The physiological functions of PRAME in normal and tumor cells are unknown, although a role in the regulation of retinoic acid signaling has been proposed. Sequence homology and structural predictions suggest that PRAME is related to the Leucine-rich repeat (LRR) family of proteins, which have diverse functions. PRAME, or "preferentially expressed antigen in melanoma", was originally identified as a gene encoding a HLA-A24 restricted antigenic peptide presented to autologous tumor-specific cytotoxic T lymphocytes derived from a patient with melanoma. PRAME is synonymous with MAPE (melanoma antigen preferentially expressed in tumors) and OIP4 (OPA-interacting protein 4), and its expression profile defines it as a cancer-testis antigen. Cancer-testis antigens (CTAs) are encoded by non-mutated genes expressed at high levels in germinal tissues and tumors, but which are absent from or detected at low levels in other tissues. PRAME may be somewhat different to other cancer-testis antigens in that it shows some expression in normal tissues such as ovary, adrenal, placenta and endometrium. The C-terminus of human PRAME (amino acids 453-509) was also identified to bind Neisseria gonorrhoeae opacity factors, in this case the OPA-P protein. Thus PRAME is also known as OIP4 (OPA interacting protein).

Pathways: Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding