Keratin Acidic (AE1) Antikörper (FITC)

Produktnummer ABIN5540046

Der Maus Monoklonal Anti--Antikörper wurde für IF, FACS und ICC validiert. Er ist geeignet, in Proben von Human, Ratte, Huhn, Rind (Kuh), Maus, Kaninchen, Affe und Schwein zu detektieren.

Kurzübersicht für Keratin Acidic (AE1) Antikörper (FITC) (ABIN5540046)

Target: Keratin Acidic (AE1)

Reaktivität: Human, Ratte, Huhn, Rind (Kuh), Maus, Kaninchen, Affe, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: FITC

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: AE1

Produktdetails

Spezifität: This antibody recognizes low molecular weight cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19(40) of the acidic family.

Aufreinigung: Protein A Chromatography

Immunogen: Human epithelial keratin.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunofluorescence: 10-20 μg/mL (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4 °C. Flow Cytometry: 0.2-1.0 μg/0.1 mL (1/200-1/1,000) (Not tested in our lab).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Antigendetails

Target: Keratin Acidic (AE1)

Andere Bezeichnung: acidic cytokeratin

Hintergrund: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.