VEGFA Antikörper (AA 288-299)

Produktnummer ABIN5539606

Dieses Anti-VEGFA-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von VEGFA in WB, IHC und ELISA. Geeignet für Human.

Kurzübersicht für VEGFA Antikörper (AA 288-299) (ABIN5539606)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser VEGFA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-VEGFA Antikörper

Bindungsspezifität: AA 288-299

Verwendungszweck: VEGFA (aa288-299)

Sequenz: RIKPHQGQHI GE

Spezifität: This antibody is expected to recognize all reported isoforms.

Kreuzreaktivität: Hund, Human, Schwein

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5 μg/mL.

Western Blot: Approx 45 kDa band observed in lysates of cell line HEK293 (calculated MW of 45.5 kDa according to NP_001020537.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Testing has shown that this antibody stains mitochondria in Mouse NIH3T3 cells at 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Andere Bezeichnung: VEGFA

Hintergrund: VEGFA, vascular endothelial growth factor A, MGC70609, MVCD1, VEGF, VPF, OTTHUMP00000016487, OTTHUMP00000016488, OTTHUMP00000165985, OTTHUMP00000165986, OTTHUMP00000165987, OTTHUMP00000224107, OTTHUMP00000224108, OTTHUMP00000224109, OTTHUMP00000224153, OT

Gen-ID: 7422

NCBI Accession: NP_001020537, NP_003367, NP_001020538, NP_001020539

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling