EGFR Antikörper (Internal Region)
Kurzübersicht für EGFR Antikörper (Internal Region) (ABIN5539519)
Target
Alle EGFR Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Güteklasse
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Bindungsspezifität
- Internal Region
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Verwendungszweck
- EGFR
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Sequenz
- QKGSHQISLD NPD
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Spezifität
- This antibody is expected to recognise isoform a (NP_005219.2) only.
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Kreuzreaktivität
- Human
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Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Isotyp
- IgG
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anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody
EGFR Reaktivität: Human WB, ELISA, IHC, FACS Wirt: Maus Monoclonal 7A6F12 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 888-1210) antibodyEGFR Reaktivität: Human WB, IHC Wirt: Maus Monoclonal C4 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 1163-1191), (C-Term) antibodyEGFR Reaktivität: Human WB, IHC (p) Wirt: Maus Monoclonal 688CT33-1-3 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibodyEGFR Reaktivität: Human, Maus, Ratte, Schwein, Affe, Rind (Kuh), Huhn, Hund, Pferd, Kaninchen, Meerschweinchen, Avian, Cat, Esel, Ziege, Hamster, Schaf WB Wirt: Ziege Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 1021-1210) antibodyEGFR Reaktivität: Human WB, IF, IP Wirt: Kaninchen Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibodyEGFR Reaktivität: Human WB, ELISA, FACS Wirt: Maus Monoclonal 5E10D3 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibodyEGFR Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer695) antibodyEGFR Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1026) antibodyEGFR Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibodyEGFR Reaktivität: Human, Ratte WB, ELISA, IHC, IP Wirt: Kaninchen Polyclonal unconjugated
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Applikationshinweise
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Western Blot: Approx 170 kDa band observed in lysates of cell line HeLa (calculated MW of 134 kDa according to NP_005219.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:128000.
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Kommentare
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Minimize freezing and thawing.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- EGFR (Epidermal Growth Factor Receptor (EGFR))
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Andere Bezeichnung
- EGFR
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Hintergrund
- EGFR, epidermal growth factor receptor, erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian , HGNC:3236, ERBB, ERBB1, mENA , avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, epidermal growth factor receptor, epidermal growth fac
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Gen-ID
- 1956
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NCBI Accession
- NP_005219
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Pathways
- NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine
Target
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