Topoisomerase II alpha Antikörper (AA 1326-1512)

Produktnummer ABIN5027278

Der Maus Monoklonal anti-Topoisomerase II alpha Antikörper wird verwendet zum Nachweis von Topoisomerase II alpha in Proben von Human. Er wurde validiert für WB und FACS.

Kurzübersicht für Topoisomerase II alpha Antikörper (AA 1326-1512) (ABIN5027278)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Klon: ABM48B4

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: AA 1326-1512

Aufreinigung: Protein G Chromatography

Immunogen: A partial length recombinant human Topo II alpha protein (amino acids 1326-1512) was used as the immunogen for this antibody.

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: WB: 1-2 μg/mL,FACS Analysis: 0.5-1 μg/10^6 cells

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: Topo II alpha

Hintergrund: Topoisomerases are essential nuclear enzymes that are involved in DNA supercoiling regulation and play key roles in transcription, replication, and chromosome segregation. Two major classes of topoisomerases (types I and II) are distinguished by the number of DNA strands that they cleave and the mechanism by which they alter the topological properties of DNA. Topo II Alpha is cell cycle-regulated and is essential for the survival of proliferating cells. It accumulates on chromatin during M-phase, a dynamic localization that is dependent on its C-terminal domain. Although Topo II alpha is the major form of Topo II responsible for decatenation, mitotic chromosome formation and chromosome segregation in proliferating cells, the contribution of the two isoforms has not yet been fully established. Topo II Alpha plays an important role in DNA synthesis and transcription and has been implicated in a variety of human cancers. It also plays a role in various cancer subtypes and acts as targets for cancer therapy, as well as biomarkers for prediction of response.

Molekulargewicht: 190 kDa

Gen-ID: 7153

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases