Sonic Hedgehog Antikörper (AA 237-399)

Produktnummer ABIN5013678

Der Kaninchen Polyklonal Anti-Sonic Hedgehog-Antikörper wurde für WB, IHC, IP und ICC validiert. Er ist geeignet, Sonic Hedgehog in Proben von Ratte zu detektieren.

Kurzübersicht für Sonic Hedgehog Antikörper (AA 237-399) (ABIN5013678)

Target: Sonic Hedgehog (SHH)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Sonic Hedgehog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-Sonic Hedgehog Antikörper

Bindungsspezifität: AA 237-399

Verwendungszweck: Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH)

Spezifität: The antibody is a rabbit polyclonal antibody raised against SHH. It has been selected for its ability to recognize SHH in immunohistochemical staining and western blotting.

Kreuzreaktivität: Human, Maus

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: SHH (Ser237-Arg399)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), ProClin

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Details zu Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Andere Bezeichnung: Hedgehog Homolog, Sonic

Hintergrund: HHG1, HLP3, HPE3, SMMCI, SHHN, SHH-N

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development