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Sonic Hedgehog Antikörper (AA 237-399)

Der Kaninchen Polyklonal Anti-Sonic Hedgehog-Antikörper wurde für WB, IHC, IP und ICC validiert. Er ist geeignet, Sonic Hedgehog in Proben von Ratte zu detektieren.
Produktnummer ABIN5013678

Kurzübersicht für Sonic Hedgehog Antikörper (AA 237-399) (ABIN5013678)

Target

Alle Sonic Hedgehog (SHH) Antikörper anzeigen
Sonic Hedgehog (SHH)

Reaktivität

  • 81
  • 57
  • 42
  • 17
  • 10
  • 6
  • 6
  • 5
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Ratte

Wirt

  • 84
  • 22
  • 9
  • 1
  • 1
Kaninchen

Klonalität

  • 76
  • 41
Polyklonal

Konjugat

  • 72
  • 10
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Sonic Hedgehog Antikörper ist unkonjugiert

Applikation

  • 89
  • 44
  • 31
  • 18
  • 18
  • 14
  • 13
  • 12
  • 11
  • 10
  • 5
  • 4
  • 3
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 15
    • 12
    • 9
    • 8
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 237-399

    Verwendungszweck

    Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH)

    Spezifität

    The antibody is a rabbit polyclonal antibody raised against SHH. It has been selected for its ability to recognize SHH in immunohistochemical staining and western blotting.

    Kreuzreaktivität

    Human, Maus

    Aufreinigung

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    SHH (Ser237-Arg399)

    Isotyp

    IgG
  • Applikationshinweise

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Kommentare

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

    Konservierungsmittel

    Dithiothreitol (DTT), ProClin

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  • Target

    Sonic Hedgehog (SHH)

    Andere Bezeichnung

    Hedgehog Homolog, Sonic

    Hintergrund

    HHG1, HLP3, HPE3, SMMCI, SHHN, SHH-N

    Pathways

    Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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