STIM1 Antikörper (C-Term)

Produktnummer ABIN500827

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch STIM1 in WB, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für STIM1 Antikörper (C-Term) (ABIN500827)

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser STIM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-STIM1 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody detects STIM1 / GOK. It is predicted to have no cross-reactivity to STIM2.

Aufreinigung: Peptide affinity chromatography

Immunogen: STIM1 antibody was raised against a 24 amino acid peptide from near the carboxy terminus of human STIM1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 1 - 2 μg/mL. Immunohisochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-STIM1 Antikörper (ABIN500827)

Wu, Hsu, Wang, Liang, Tsai, Yen, Li, Chiu, Chang, Huang, Lin, Yoshioka: "A Novel Strategy for TNF-Alpha Production by 2-APB Induced Downregulated SOCE and Upregulated HSP70 in O. tsutsugamushi-Infected Human Macrophages." in: PLoS ONE, Vol. 11, Issue 7, pp. e0159299, (2017) (PubMed).

Misceo, Holmgren, Louch, Holme, Mizobuchi, Morales, De Paula, Stray-Pedersen, Lyle, Dalhus, Christensen, Stormorken, Tjønnfjord, Frengen: "A dominant STIM1 mutation causes Stormorken syndrome." in: Human mutation, Vol. 35, Issue 5, pp. 556-64, (2014) (PubMed).

Details zu STIM1

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Andere Bezeichnung: STIM1 / GOK

Hintergrund: In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least two isoforms of STIM1 are known to exist, this antibody will detect only the larger form.Synonyms: Stromal interaction molecule 1

Gen-ID: 6786

NCBI Accession: NP_003147

Pathways: T-Zell Rezeptor Signalweg, BCR Signaling