STIM1 Antikörper (C-Term)

Produktnummer ABIN192394

Dieser Maus Monoklonal Antikörper detektiert spezifisch STIM1 in WB, IP, IHC (p) und ICC. Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für STIM1 Antikörper (C-Term) (ABIN192394)

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser STIM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Klon: CDN3H4

Produktdetails anti-STIM1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-STIM1 Purified

Kreuzreaktivität (Details): Human, Mouse, Rat

Produktmerkmale: The mouse monoclonal antibody CDN3H4 reacts with a cytoplasmic epitope of human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Synthetized peptide (C-terminal cytoplasmic part of STIM1).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry: Methanol-aceton fixation, positive control: HeLa human cervix carcinoma cell line.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL.
Western blotting: Recommended dilution: 1 μg/mL, positive control: RBL rat basophilic leukemia cell line, both reducing and non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-STIM1 Antikörper (ABIN192394)

Hájková, Bugajev, Dráberová, Vinopal, Dráberová, Janá?ek, Dráber, Dráber: "STIM1-directed reorganization of microtubules in activated mast cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 2, pp. 913-23, (2011) (PubMed).

Ng, Ramduny, Airey, Singer, Keller, Shen, Tian, Valencik, Hume: "Orai1 interacts with STIM1 and mediates capacitative Ca2+ entry in mouse pulmonary arterial smooth muscle cells." in: American journal of physiology. Cell physiology, Vol. 299, Issue 5, pp. C1079-90, (2010) (PubMed).

Details zu STIM1

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Andere Bezeichnung: STIM1

Hintergrund: Stromal interaction molecule 1,STIM1 (stromal interacting molecule, also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.,GOK, TAM1, STRMK

Gen-ID: 6786

UniProt: G0XQ39

Pathways: T-Zell Rezeptor Signalweg, BCR Signaling