DDX58 Antikörper

Produktnummer ABIN500606

Der Kaninchen Polyklonal Anti-DDX58-Antikörper wurde für WB, IHC (p) und EIA validiert. Er ist geeignet, DDX58 in Proben von Human, Maus und Ratte zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für DDX58 Antikörper (ABIN500606)

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDX58 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-DDX58 Antikörper

Spezifität: This antibody detects DEAD box protein 58.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse, rat

Aufreinigung: Peptide affinity chromatography

Immunogen: RIG-1 antibody was raised against human GST-tagged RIG-1 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-DDX58 Antikörper (ABIN500606)

Korolowicz, Iyer, Czerwinski, Suresh, Yang, Padmanabhan, Sheri, Pandey, Skell, Marquis, Kallakury, Tucker, Menne: "Antiviral Efficacy and Host Innate Immunity Associated with SB 9200 Treatment in the Woodchuck Model of Chronic Hepatitis B." in: PLoS ONE, Vol. 11, Issue 8, pp. e0161313, (2017) (PubMed).

Gan, Hao, Idell, Tang: "Transcription Factor Runx3 Is Induced by Influenza A Virus and Double-Strand RNA and Mediates Airway Epithelial Cell Apoptosis." in: Scientific reports, Vol. 5, pp. 17916, (2016) (PubMed).

Details zu DDX58

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Andere Bezeichnung: DDX58

Hintergrund: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG-1) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG-1 activates the kinases TBK1 and IKKepsilon through the adaptor protein IPS-1. These kinases then phosphorylate the transcription factors IRF-3 and IRF-7 which are essential for the expression of type-I interferons. RIG-1 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.Synonyms: DEAD box protein 58, DKFZp434J1111, DKFZp686N19181, FLJ13599, Probable ATP-dependent RNA helicase DDX58, RIG-1, RIG-I, Retinoic acid-inducible gene 1 protein

Gen-ID: 23586

UniProt: O95786

Pathways: Activation of Innate immune Response, Hepatitis C