Cyclin D1 Antikörper (AA 61-110) (AbBy Fluor® 750)

Produktnummer ABIN5000805

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Cyclin D1 in WB, FACS, IF (cc) und IF (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Kaninchen und Schaf.

Kurzübersicht für Cyclin D1 Antikörper (AA 61-110) (AbBy Fluor® 750) (ABIN5000805)

Target: Cyclin D1 (CCND1)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Schaf

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin D1 Antikörper ist konjugiert mit AbBy Fluor® 750

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-Cyclin D1 Antikörper (AbBy Fluor® 750)

Bindungsspezifität: AA 61-110

Kreuzreaktivität: Rind (Kuh), Human, Maus, Kaninchen, Ratte, Schaf

Homologie: Dog

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Cyclin D1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Cyclin D1

Target: Cyclin D1 (CCND1)

Andere Bezeichnung: Cyclin D1

Hintergrund:

Synonyms: cD1, CycD1, Cyl-1, PRAD1, bcl-1, AI32739, G1/S-specific cyclin-D1, Ccnd1

Background: Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity).

Gen-ID: 12443

UniProt: P25322

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, ER-Nucleus Signaling