CHEK2 Antikörper (pSer516) (AbBy Fluor® 680)

Produktnummer ABIN4999985

Dieses Anti-CHEK2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CHEK2 in WB. Geeignet für Human, Maus und Ratte.

Kurzübersicht für CHEK2 Antikörper (pSer516) (AbBy Fluor® 680) (ABIN4999985)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHEK2 Antikörper ist konjugiert mit AbBy Fluor® 680

Applikation: Western Blotting (WB)

Produktdetails anti-CHEK2 Antikörper (AbBy Fluor® 680)

Bindungsspezifität: pSer516

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Dog,Pig,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CHK2 around the phosphorylation site of ser516

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Andere Bezeichnung: CHK2

Hintergrund:

Synonyms: Chk2 Ser516, bA444G7, CHK2 checkpoint homolog, CHK2_HUMAN, Serine/threonine-protein kinase Chk2, CDS 1, CDS1, Checkpoint kinase 2, Checkpoint like protein CHK2, Chek 2, Chek2, Chk 2, CHK2 checkpoint homolog S. pombe, CHK2 checkpoint homolog, HuCds 1, HuCds1, LFS 2, LFS2, PP1425, RAD 53, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2.

Background: Chk2 is a serine/threonine kinase involved in the control of cell cycle checkpoints, and may also participate in transduction of the DNA damage and replicational stress signals. Chk2 is the mammalian ortholog of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of Chk2 contains a series of seven serine and threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases. Indeed, after DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain. Chk2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, preventing the entry into mitosis. This kinase may have a role in meiosis as well. Kinase activity is up regulated by autophosphorylation and the protein is rapidly phosphorylated in response to DNA damage and to replication block.

Gen-ID: 11200

Pathways: p53 Signalweg, Apoptose, Zellzyklus