CD3 Antikörper

Produktnummer ABIN498498

Dieses Anti-CD3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CD3 in WB und IHC (p). Geeignet für Human.

Kurzübersicht für CD3 Antikörper (ABIN498498)

Target: CD3

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CD3 Antikörper

Spezifität: This antibody detects endogenous levels of CD3-epsilon protein. (region surrounding Asp71)

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD3

Target: CD3

Andere Bezeichnung: CD3

Hintergrund: The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (Cluster of Differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (gamma-epsilon), a heterodimer of delta and epsilon chains (delta-epsilon) and a homodimer of two zeta chains (zeta-zeta) or a heterodimer of zeta and eta chains (zeta-eta). The zeta and eta chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The gamma, epsilon and delta chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the zeta chain contains three consecutive copies of the same motif.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Molekulargewicht: approx. 28 kDa

Gen-ID: 916

NCBI Accession: NP_000724

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway