Mre11 Antikörper

Produktnummer ABIN498267

Produktdetails anti-Mre11 Antikörper

Target: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Mre11 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of MRE11 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography using epitope-specific immunogen

Reinheit: > 95 % (by SDS-PAGE)

Anwendungsinformationen

Applikationshinweise: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu Mre11

Target: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Andere Bezeichnung: MRE11 (MRE11A Produkte)

Synonyme: wu:faa63d12 antikoerper, zgc:64018 antikoerper, Mre11 antikoerper, Mre11b antikoerper, ATLD antikoerper, HNGS1 antikoerper, MRE11 antikoerper, MRE11B antikoerper, MRE11 homolog A, double strand break repair nuclease antikoerper, MRE11 homolog, double strand break repair nuclease antikoerper, MRE11A homolog A, double strand break repair nuclease antikoerper, mre11a antikoerper, MRE11 antikoerper, Mre11a antikoerper

Hintergrund: DNA double-strand breaks are generated by ionizing radiation and endogenously produced radicals, and they often are repaired through the RAD52 homologous recombination pathway. The RAD52 family includes RAD51, RAD52, RAD54, RAD54B and MRE11 genes. Rad51 and Rad52 proteins perform the key steps in homologous recombination (HR), including the search for DNA homology and strand exchange, through similar mechanisms. Mre11 functions in both non-homologous end joining, and meiotic HR, and it is essential in mitosis for chromosome maintenance. Rad54 belongs to the SWI2/SNF2 subfamily of ATPases, which includes the DNA helicases involved in replication, recombination, and repair, as it contains seven amino acid sequence motifs that are largely conserved. Rad54 ATPase activity is dependent on double-stranded (ds) DNA, and the ATPase activity of Rad54 is not absolutely required for its DNA repair function, suggesting that these activities occur at distinct regions of the molecule. RAD54B is significantly homologous to the RAD54 recombination gene. Expression of RAD54B is highest in testis and spleen, which are active in both meiotic and mitotic recombination.Synonyms: Double-strand break repair protein MRE11A, HNGS1, MRE11 homolog 1, MRE11 meiotic recombination 11 homolog A, MRE11A

Molekulargewicht: approx. 80 kDa

Gen-ID: 4361

NCBI Accession: NP_005582

UniProt: P49959

Pathways: DNA Reparatur