Lamin B2 Antikörper (AA 221-460)

Produktnummer ABIN497934

Dieses Anti-Lamin B2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Lamin B2 in WB und IHC (p). Geeignet für Human.

Kurzübersicht für Lamin B2 Antikörper (AA 221-460) (ABIN497934)

Target: Lamin B2 (LMNB2)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Lamin B2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Lamin B2 Antikörper

Bindungsspezifität: AA 221-460

Spezifität: Recognizes Lamin B2.
Cellular Localization: Nucleus inner membrane, Lipid-anchor, Nucleoplasmic side

Aufreinigung: Antigen-Affinity Chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 221 and 460 of Lamin B2

Anwendungsinformationen

Applikationshinweise: Western blotting: 1/500-1/3000. Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.1 M Tris, 0.1 M Glycine, 10 % Glycerol and 0.01 % Thimerosal as preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at -20 °C.

Details zu Lamin B2

Target: Lamin B2 (LMNB2)

Andere Bezeichnung: Lamin-B2 (LMNB2)

Hintergrund: An important part of the cell nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. Lamin A, Lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. Lamin B1 and Lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of a-helices and a carboxyterminal globular domain. Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus. Besides interactions with the nuclear membrane and other intermediate filaments, lamins interact with the nuclear chromatin. Eukaryotic chromatin is organised into loops, which are attached to the nuclear matrix. This organisation is thought to contribute to compaction of the chromatin and regulation of gene expression. Lamins, as part of the nuclear matrix, may be involved in these processes since chromatin binding sites have been detected in both A- and B-type Lamins.Synonyms: LMN2, LMNB, LMNB-2, LMNB2, Lamin-B2, Nuclear Envelope Marker

Gen-ID: 84823, 9606

UniProt: Q03252

Pathways: Apoptose, Caspase Kaskade in der Apoptose