AGO1 Antikörper (N-Term) (HRP)

Produktnummer ABIN4966093

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch AGO1 in WB. Er zeigt eine Reaktivität gegenüber Arabidopsis thaliana und Nicotiana benthamiana.

Kurzübersicht für AGO1 Antikörper (N-Term) (HRP) (ABIN4966093)

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reaktivität: Arabidopsis thaliana, Nicotiana benthamiana

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AGO1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB)

Produktdetails anti-AGO1 Antikörper (HRP)

Bindungsspezifität: N-Term

Kreuzreaktivität (Details): Not reactive in: Chlamydomonas reinhardtii

Homologie: Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera

Immunogen: N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379, TAIR: At1g48410.

Anwendungsinformationen

Applikationshinweise: 1: 1000 with enhanced ECL (WB)

Kommentare:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Informationen zur Lagerung: Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Details zu AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Andere Bezeichnung: argonaute 1 (AGO1)

Hintergrund: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).This antibody is directly conjugated to Horseradish Peroxidase (HRP).

Molekulargewicht: 116.4 | 130 kDa

UniProt: O04379

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization