AGO1 Antikörper (N-Term)

Produktnummer ABIN488536

Produktdetails anti-AGO1 Antikörper

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Bindungsspezifität: N-Term

Reaktivität: Arabidopsis thaliana, Nicotiana benthamiana

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AGO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: Antibody binds microRNA and tasiRNAs, preference for 21ntmiRNAs with 5'U

Kreuzreaktivität (Details): Not reactive in: Chlamydomonas reinhardtii

Homologie: Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Solanum tuberosum, Zea mays, Vitis vinifera

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 116.4 / 130 kDa
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition.

Aufreinigung: serum

Immunogen: N-terminal peptide of Arabidopsis thaliana AGO1 O04379, At1g48410

Anwendungsinformationen

Applikationshinweise: 1: 5000 - 1: 10 000 (WB)

Kommentare:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water.

Handhabung: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Referenzen für anti-AGO1 Antikörper (ABIN488536)

Speth, Willing, Rausch, Schneeberger, Laubinger: "RACK1 scaffold proteins influence miRNA abundance in Arabidopsis." in: The Plant journal : for cell and molecular biology, Vol. 76, Issue 3, pp. 433-45, (2013) (PubMed).

Details zu AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Andere Bezeichnung: AGO1 (EIF2C1 Produkte)

Synonyme: Ago antikoerper, Ago-1 antikoerper, Ago1 antikoerper, CG6671 antikoerper, Dm Ago1 antikoerper, Dmel\\CG6671 antikoerper, MRE20 antikoerper, ago1 antikoerper, ago1-1 antikoerper, anon-WO0257455.29 antikoerper, dAGO1 antikoerper, dAgo1 antikoerper, l(2)04845 antikoerper, l(2)4845 antikoerper, l(2)k00208 antikoerper, l(2)k08121 antikoerper, GB12654 antikoerper, dsim_GLEANR_9718 antikoerper, DsimGD25729 antikoerper, GD25729 antikoerper, EIF2C1 antikoerper, EIF2C antikoerper, GERP95 antikoerper, Q99 antikoerper, Eif2c1 antikoerper, ARGONAUTE 1 antikoerper, T1N15.2 antikoerper, T1N15_2 antikoerper, Argonaute-1 antikoerper, protein argonaute-2 antikoerper, argonaute 1 antikoerper, Argonaute 1 antikoerper, protein argonaute-1 antikoerper, argonaute 1, RISC catalytic component antikoerper, argonaute RISC catalytic subunit 1 antikoerper, Stabilizer of iron transporter SufD / Polynucleotidyl transferase antikoerper, AGO1 antikoerper, LOC552062 antikoerper, ago1 antikoerper, LOC659936 antikoerper, Dsim\AGO1 antikoerper, Ago1 antikoerper, LOC100386910 antikoerper, LOC100482671 antikoerper, LOC475337 antikoerper

Hintergrund: AGI Code: At1g48410
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Molekulargewicht: expected: 116.4 kDa, apparent: 130 kDa

UniProt: O04379

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulatorische RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization