UHRF1 Antikörper (C-Term)

Produktnummer ABIN3029442

Dieses Anti-UHRF1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von UHRF1 in WB und ELISA. Geeignet für Maus.

Kurzübersicht für UHRF1 Antikörper (C-Term) (ABIN3029442)

Target: UHRF1 (Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UHRF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-UHRF1 Antikörper

Bindungsspezifität: C-Term

Aufreinigung: Antigen affinity purified

Immunogen: This mouse Uhrf1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 596-628 amino acids from the C-terminal region of mouse Uhrf1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the Uhrf1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000-2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the Uhrf1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu UHRF1

Target: UHRF1 (Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1))

Andere Bezeichnung: Uhrf1

Hintergrund: Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD- type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.

UniProt: Q8VDF2