SMAD3 Antikörper (AA 157-191)

Produktnummer ABIN3032624

Der Kaninchen Polyklonal Anti-SMAD3-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, SMAD3 in Proben von Ratte zu detektieren.

Kurzübersicht für SMAD3 Antikörper (AA 157-191) (ABIN3032624)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-SMAD3 Antikörper

Bindungsspezifität: AA 157-191

Aufreinigung: Antigen affinity purified

Immunogen: This mouse Smad3 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 157-191 amino acids from the Central region of mouse Smad3.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the Smad3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the Smad3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Andere Bezeichnung: Smad3

Hintergrund: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity). [UniProt]

UniProt: Q8BUN5

Pathways: Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie