SMAD3 Antikörper (AA 127-156)

Produktnummer ABIN3032620

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SMAD3 in WB, ELISA, IHC und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für SMAD3 Antikörper (AA 127-156) (ABIN3032620)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-SMAD3 Antikörper

Bindungsspezifität: AA 127-156

Kreuzreaktivität (Details): Expected species reactivity: Mouse, Rat, Pig, Chicken

Aufreinigung: Antigen affinity purified

Immunogen: A portion of amino acids 127-156 from the human protein was used as the immunogen for this SMAD3 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the SMAD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:10-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the SMAD3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Andere Bezeichnung: SMAD3

Hintergrund: SMAD3 is a receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. [UniProt]

UniProt: P84022

Pathways: Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie