Sonic Hedgehog Antikörper (N-Term)

Produktnummer ABIN3032696

Der Kaninchen Polyklonal Anti-Sonic Hedgehog-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, Sonic Hedgehog in Proben von Maus und Ratte zu detektieren.

Kurzübersicht für Sonic Hedgehog Antikörper (N-Term) (ABIN3032696)

Target: Sonic Hedgehog (SHH)

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Sonic Hedgehog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Sonic Hedgehog Antikörper

Bindungsspezifität: N-Term

Aufreinigung: Antigen affinity purified

Immunogen: This mouse Shh antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 58-91 amino acids from the N-terminal region of mouse Sonic hedgehog.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the Shh antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The 45 kDa precursor protein autocleaves into 27 kDa amino and 19 kDa carboxy fragments.\. Western blot: 1:1000-2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the Shh antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Andere Bezeichnung: Shh

Hintergrund: Sonic hedgehog intercellular signaling is essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO. [UniProt]

UniProt: Q62226

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development