PAK1 Antikörper (AA 401-430)

Produktnummer ABIN3032114

Der Kaninchen Polyklonal Anti-PAK1 Antikörper wurde für WB, IHC, ELISA und IF validiert. Er ist geeignet, PAK1 in Proben von Human zu detektieren.

Kurzübersicht für PAK1 Antikörper (AA 401-430) (ABIN3032114)

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-PAK1 Antikörper

Bindungsspezifität: AA 401-430

Kreuzreaktivität (Details): Expected species reactivity: Mouse, Rat, Bovine, Rabbit, Xenopus

Aufreinigung: Antigen affinity purified

Immunogen: A portion of amino acids 401-430 from the human protein was used as the immunogen for this PAK1 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the PAK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:10-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the PAK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Andere Bezeichnung: PAK1

Hintergrund: PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. The protein interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. The protein is autophosphorylated when activated by CDC42/p21. Structurally, the PAK1 contains 1 CRIB domain.

UniProt: Q13153

Pathways: MAPK Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis