LKB1 Antikörper (AA 14-44)

Produktnummer ABIN3032540

Der Kaninchen Polyklonal Anti-LKB1-Antikörper wurde für WB, ELISA, IHC, IF und FACS validiert. Er ist geeignet, LKB1 in Proben von Human zu detektieren.

Kurzübersicht für LKB1 Antikörper (AA 14-44) (ABIN3032540)

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LKB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-LKB1 Antikörper

Bindungsspezifität: AA 14-44

Kreuzreaktivität (Details): Expected species reactivity: Mouse, Rat, Chicken, Xenopus

Aufreinigung: Purified

Immunogen: A portion of amino acids 14-44 from the human protein was used as the immunogen for this LKB1 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the LKB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (Paraffin): 1:50-1:100,Western blot: 1:1000,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the LKB1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu LKB1

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Andere Bezeichnung: LKB1

Hintergrund: LKB1 is a tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53, the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. In vein endothelial cells, inhibits PI3K/Akt signaling activity and thus induces apoptosis in response to the oxidant peroxynitrite (in vitro). Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair. [UniProt]

UniProt: Q15831

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt