Cyclin D1 Antikörper (AA 68-97)

Produktnummer ABIN3030588

Dieses Anti-Cyclin D1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Cyclin D1 in WB, IHC, ELISA und IF. Geeignet für Human und Maus.

Kurzübersicht für Cyclin D1 Antikörper (AA 68-97) (ABIN3030588)

Target: Cyclin D1 (CCND1)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin D1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-Cyclin D1 Antikörper

Bindungsspezifität: AA 68-97

Kreuzreaktivität (Details): Expected species reactivity: Bovine

Aufreinigung: Antigen affinity purified

Immunogen: A portion of amino acids 68-97 from the human protein was used as the immunogen for this Cyclin D1 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the Cyclin D1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the Cyclin D1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu Cyclin D1

Target: Cyclin D1 (CCND1)

Hintergrund: CCND1 is the regulatory component of the Cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. CyclinD-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. [UniProt]

UniProt: P24385

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, ER-Nucleus Signaling