ASK1 Antikörper (AA 944-973)

Produktnummer ABIN3031853

Der Kaninchen Polyklonal Anti-ASK1-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, ASK1 in Proben von Human zu detektieren.

Kurzübersicht für ASK1 Antikörper (AA 944-973) (ABIN3031853)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ASK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-ASK1 Antikörper

Bindungsspezifität: AA 944-973

Kreuzreaktivität (Details): Expected species reactivity: Mouse

Aufreinigung: Antigen affinity purified

Immunogen: A portion of amino acids 944-973 from the human protein was used as the immunogen for this ASK1 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the ASK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the ASK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Andere Bezeichnung: ASK1

Hintergrund: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. ASK1/MAPKKK5/MAP3K5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that transcript is abundantly expressed in human heart and pancreas. The MAP3K5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAP3K5 does not activate MAPK/ERK.

UniProt: Q99683

Pathways: MAPK Signalweg, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response