ATG7 Antikörper (AA 284-313)

Produktnummer ABIN3029987

Der Kaninchen Polyklonal Anti-ATG7-Antikörper wurde für WB, IHC und ELISA validiert. Er ist geeignet, ATG7 in Proben von Human und Maus zu detektieren.

Kurzübersicht für ATG7 Antikörper (AA 284-313) (ABIN3029987)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-ATG7 Antikörper

Bindungsspezifität: AA 284-313

Kreuzreaktivität (Details): Expected species reactivity: Rat, Chicken

Aufreinigung: Purified

Immunogen: A portion of amino acids 284-313 from the human protein was used as the immunogen for this APG7 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the APG7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the APG7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Andere Bezeichnung: ATG7 (APG7)

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagie