SIRT1 Antikörper (AA 566-601)

Produktnummer ABIN3032583

Dieses Anti-SIRT1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SIRT1 in WB, IHC, ELISA und IF. Geeignet für Human und Maus.

Kurzübersicht für SIRT1 Antikörper (AA 566-601) (ABIN3032583)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIRT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-SIRT1 Antikörper

Bindungsspezifität: AA 566-601

Aufreinigung: Antigen affinity purified

Immunogen: A portion of amino acids 566-601 of the mouse protein was used as the immunogen for the SIRT1 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the SIRT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (Paraffin): 1:25,Immunofluorescence: 1:25,Western blot: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at 4°C for up to one month. For long term, aliquot the SIRT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Andere Bezeichnung: SIRT1

Hintergrund: SIRT1 is a NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. [UniProt]

UniProt: Q923E4

Pathways: MAPK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling