PARP1 Antikörper (AA 590-618)

Produktnummer ABIN3032268

Der Kaninchen Polyklonal anti-PARP1 Antikörper (ABIN3032268) detektiert spezifisch PARP1 in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für PARP1 Antikörper (AA 590-618) (ABIN3032268)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PARP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-PARP1 Antikörper

Bindungsspezifität: AA 590-618

Kreuzreaktivität (Details): Expected species reactivity: Bovine

Aufreinigung: Antigen affinity purified

Immunogen: A portion of amino acids 590-618 from the human protein was used as the immunogen for this PARP 1 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the PARP 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the PARP 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Andere Bezeichnung: PARP

Hintergrund: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. [UniProt]

UniProt: P09874

Pathways: Apoptose, Caspase Kaskade in der Apoptose, DNA Reparatur, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location