Topoisomerase II alpha Antikörper (AA 1352-1493)

Produktnummer ABIN4949920

Der Maus Monoklonal anti-Topoisomerase II alpha Antikörper wird verwendet zum Nachweis von Topoisomerase II alpha in Proben von Human. Er wurde validiert für WB, IF, FACS und IHC (p).

Kurzübersicht für Topoisomerase II alpha Antikörper (AA 1352-1493) (ABIN4949920)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: TOP2A-1362

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: AA 1352-1493

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids 1352-1493 from the human protein were used as the immunogen for this TOP2A antibody.

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/10^6 cells in 0.1ml,IF: 1-2 μg/mL,Western blot: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the TOP2A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: TOP2A / Topoisomerase II alpha

Hintergrund: This mAb recognizes an ~170  kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Gen-ID: 7153

Pathways: Zellzyklus, Mitotic G1-G1/S Phases