Topoisomerase II alpha Antikörper (AA 1352-1493)

Produktnummer ABIN4949920

Der Maus Monoklonal Anti-Topoisomerase II alpha-Antikörper wurde für WB, IF, FACS und IHC (p) validiert. Er ist geeignet, Topoisomerase II alpha in Proben von Human zu detektieren.

Kurzübersicht für Topoisomerase II alpha Antikörper (AA 1352-1493) (ABIN4949920)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: TOP2A-1362

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: AA 1352-1493

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids 1352-1493 from the human protein were used as the immunogen for this TOP2A antibody.

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/10^6 cells in 0.1ml,IF: 1-2 μg/mL,Western blot: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the TOP2A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: TOP2A / Topoisomerase II alpha

Hintergrund: This mAb recognizes an ~170  kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Gen-ID: 7153

Pathways: Zellzyklus, Mitotic G1-G1/S Phases