Thymidine Phosphorylase Antikörper

Produktnummer ABIN4949902

Der Maus-Monoklonal Anti-Thymidine Phosphorylase-Antikörper wurde für WB, IP und IHC (p) validiert. Er ist geeignet zum Nachweis von Thymidine Phosphorylase in Proben aus Human, Ratte und Maus. }

Kurzübersicht für Thymidine Phosphorylase Antikörper (ABIN4949902)

Target: Thymidine Phosphorylase (TYMP)

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Thymidine Phosphorylase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM322

Produktdetails anti-Thymidine Phosphorylase Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Human recombinant full length protein was used as the immunogen for this Thymidine Phosphorylase antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thymidine Phosphorylase antibody to be titered up or down for optimal performance.\. IP: 1-2 μg/500 μg lysate,IHC (FFPE): 1-2 μg/mL for 30 min at RT,WB: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Thymidine Phosphorylase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Thymidine Phosphorylase

Target: Thymidine Phosphorylase (TYMP)

Andere Bezeichnung: Thymidine Phosphorylase / PD-ECGF

Hintergrund: Recognizes a 485 amino acid protein (55 kDa monomer / 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), also called Thymidine Phosphorylase (TP, Tymp) or Gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2