CD35 Antikörper

Produktnummer ABIN4949510

Dieses Anti-CD35-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD35 in FACS, IF und IHC (p). Geeignet für Human, Pavian und Affe.

Kurzübersicht für CD35 Antikörper (ABIN4949510)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reaktivität: Human, Pavian, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD35 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: CR1-802

Produktdetails anti-CD35 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the CD35 antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CD35 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the CD35 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Andere Bezeichnung: CD35

Hintergrund: Recognizes a protein of 210-220  kDa, which is identified as the complement receptor 1 (CR1)/CD35. This mAb does not block CR1 activity. It is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This mAb labels follicular dendritic cells and follicular dendritic cell sarcoma.

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity