Rekombinanter ACTH Antikörper (AA 1-24)

Produktnummer ABIN4949428

Der Maus Monoklonal Anti-ACTH-Antikörper wurde für IHC (p) validiert. Er ist geeignet, ACTH in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für Rekombinanter ACTH Antikörper (AA 1-24) (ABIN4949428)

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ACTH Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: R57

Produktdetails anti-ACTH Antikörper

Bindungsspezifität: AA 1-24

Aufreinigung: Protein G affinity chromatography

Immunogen: An N-terminal peptide (within AA 1-24) from the Synacthen/ACTH region of human POMC was used as the immunogen for this recombinant ACTH antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the recombinant ACTH antibody for each application should be determined by the researcher.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant ACTH antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu ACTH

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Andere Bezeichnung: ACTH / POMC

Hintergrund: ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This mAb is specific to Synacthen (aa1-24 of ACTH), does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity