Rekombinanter S100B Antikörper

Produktnummer ABIN4949395

Der Kaninchen Monoklonal Anti-S100B-Antikörper wurde für WB, IF, FACS und IHC (p) validiert. Er ist geeignet, S100B in Proben von Human, Ratte, Maus und Rind (Kuh) zu detektieren.

Kurzübersicht für Rekombinanter S100B Antikörper (ABIN4949395)

Target: S100B (S100 Calcium Binding Protein B (S100B))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Maus, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser S100B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: S100B-1706R

Produktdetails anti-S100B Antikörper

Aufreinigung: Protein A affinity chromatography

Immunogen: Full length human recombinant S100B protein was used as the immunogen for this recombinant S100B antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the recombinant S100B antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant S100B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu S100B

Target: S100B (S100 Calcium Binding Protein B (S100B))

Andere Bezeichnung: S100B

Hintergrund: S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteine