Rekombinanter Keratin Acidic (AE1) Antikörper

Produktnummer ABIN4949246

Dieses Anti--Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von in IF, WB, FACS und IHC (p). Geeignet für Human.

Kurzübersicht für Rekombinanter Keratin Acidic (AE1) Antikörper (ABIN4949246)

Target: Keratin Acidic (AE1)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: KRTL-1577R

Produktdetails

Aufreinigung: Protein A affinity chromatography

Immunogen: An amino acid sequence common to acidic/type I keratins (1) was used as the immunogen for the recombinant Acidic Cytokeratin antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Titering of the recombinant Acidic Cytokeratin antibody may be required for optimal performance.

1. This antibody will detect cytokeratins -10, -14, -15, -16, -19.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
3. Required HIER: boil tissue sections in 10  mM citrate buffer,  pH 6, for 10-20 min followed by cooling at RT for 20 min.\. Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (3),Prediluted IHC only format: incubate for 30 min at RT (2),FACS: 0.5-1 μg/million cells in 0.1ml,WB: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant Acidic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Antigendetails

Target: Keratin Acidic (AE1)

Andere Bezeichnung: Acidic Cytokeratin

Hintergrund: There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki]