Aurora A Antikörper (C-Term)

Produktnummer ABIN492609

Produktdetails anti-Aurora A Antikörper

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Aurora A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Spezifität: Recognizes Aurora-A/B (C-term).

Produktmerkmale: Synonyms: Aurora kinase A, Aurora A, Aurora B, AURKA, AURKB, AURA, AURB, BTAK, STK6, ARK1, STK15,AIK, AIK2, AIM1, ARK2, STK12

Aufreinigung: Size Exclusion Chromatography.

Bestandteile: incl. positive Control

Immunogen: Peptide conjugated to hemocyaninEpitope: C-terminus.

Klon: 5F11

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunobotting (Western Blot): 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking buffer and blotincubation buffer. Included Postitive Control: Cell lysate from untreated SW620 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Positive Control: Cell lysate from untreated SW620 cells. Formulation: Lyophilized Cell lysate from Serum starved SW620 cells. Stability: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The Positive Control lysate is recommended for Immunoblot applications. 20µl of PositiveControl correspond to ca. 20. 000 cells. Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch. The Lyophilized cell lysates contain SDS and are not recommended for applications withnative proteins such as Immunoprecipitation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: PBS / 0.09 % Sodium Azide / PEG and Sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -20 °C to -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Andere Bezeichnung: Aurora Kinase A (AURKA Produkte)

Hintergrund: Aurora proteins are members of a serine/threonine kinase family. They play a crucial role in mitosis by regulating chromosome segregation and cytokinesis. There are three forms of Aurora proteins in mammalian cells: AuroraA, B and C. Aurora-A (Aurora-2, STK6, ARK1, Aurora/IPL-1 related kinase) associates with centrosomes and microtubules during mitosis. Phosphorylation of a threonine residue within the activation loop of the catalytic domain lead to activation of Aurora-A. AuroraB (Aurora-1) is responsible for chromatin modification and histone H3 phosphporylation.Synonyms: AIK, AIK2, AIM1, ARK1, ARK2, AURA, AURB, AURKA, AURKB, Aurora A, Aurora B, Aurora kinase A, BTAK, STK12, STK15, STK6

Gen-ID: 6790

UniProt: O14965

Pathways: Zellzyklus, Asymmetric Protein Localization