Aurora A Antikörper (N-Term)

Produktnummer ABIN492608

Produktdetails anti-Aurora A Antikörper

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Aurora A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: Recognizes Aurora-A (N-term).

Produktmerkmale: Synonyms: AURKA, AIK1, ARK1, AURA, BTAK, STK15, STK6, Aurora/IPL1-related kinase 1, AURORA2,Serine/threonine-protein kinase 6, Serine/threonine kinase 15, Breast tumor-amplifiedkinase

Aufreinigung: Size Exclusion Chromatography.

Bestandteile: incl. positive Control

Immunogen: Peptide conjugated to hemocyaninEpitope: N-terminus.

Klon: 7F12

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunobotting (Western Blot): 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking buffer and blotincubation buffer. Included Postitive Control: Cell Lysate from untreated A431 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Positive Control: Cell Lysate from untreated A431 cells. Formulation: Lyophilized Cell lysate from Serum starved A431 cells. Stability: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The Positive Control lysate is recommended for Immunoblot applications. 20µl of PositiveControl correspond to ca. 20. 000 cells. Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch. The Lyophilized cell lysates contain SDS and are not recommended for applications withnative proteins such as Immunoprecipitation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: PBS / 0.09 % Sodium Azide / PEG and Sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -20 °C to -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Andere Bezeichnung: Aurora Kinase A (AURKA Produkte)

Synonyme: AIK antikoerper, ARK1 antikoerper, AURA antikoerper, AURORA2 antikoerper, BTAK antikoerper, PPP1R47 antikoerper, STK15 antikoerper, STK6 antikoerper, STK7 antikoerper, AIRK1 antikoerper, AU019385 antikoerper, AW539821 antikoerper, Ark1 antikoerper, Aurora-A antikoerper, Ayk1 antikoerper, IAK antikoerper, IAK1 antikoerper, Stk6 antikoerper, 87A7-9/2 antikoerper, Aur-A antikoerper, AurA antikoerper, CG3068 antikoerper, Dmel\\CG3068 antikoerper, aur-A antikoerper, aurA antikoerper, ck10 antikoerper, ck[10] antikoerper, l(3)87Ac antikoerper, l(3)ck10 antikoerper, ARK-1 antikoerper, Eg2 antikoerper, aik antikoerper, ark1 antikoerper, aur-a antikoerper, aura antikoerper, aurka-a antikoerper, aurora2 antikoerper, btak antikoerper, stk15 antikoerper, stk6 antikoerper, stk7 antikoerper, pEg2 antikoerper, wu:fb37c10 antikoerper, wu:fc02c02 antikoerper, zgc:100912 antikoerper, AURKA antikoerper, aurka antikoerper, aurora kinase A antikoerper, aurora A antikoerper, aurora kinase A L homeolog antikoerper, hypothetical protein antikoerper, aurora kinase A S homeolog antikoerper, AURKA antikoerper, Aurka antikoerper, aurA antikoerper, aurka.L antikoerper, aurka antikoerper, Aurora-A antikoerper, aurka.S antikoerper

Hintergrund: Aurora proteins are members of a serine/threonine kinase family. They play a crucial role in mitosis by regulating chromosome segregation and cytokinesis. There are three forms of Aurora proteins in mammalian cells: AuroraA, B and C. Aurora-A (Aurora-2, STK6, ARK1, Aurora/IPL-1 related kinase) associates with centrosomes and microtubules during mitosis. Phosphorylation of a threonine residue within the activation loop of the catalytic domain lead to activation of Aurora-A. AuroraB (Aurora-1) is responsible for chromatin modification and histone H3 phosphporylation.Synonyms: AIK1, ARK1, AURA, AURKA, AURORA2, Aurora/IPL1-related kinase 1, BTAK, Breast tumor-amplified kinase, STK15, STK6, Serine/threonine kinase 15, Serine/threonine-protein kinase 6

Gen-ID: 6790

UniProt: O14965

Pathways: Zellzyklus, Asymmetric Protein Localization