EGFR Antikörper (pTyr869) (FITC)

Produktnummer ABIN492341

Der Maus Monoklonal anti-EGFR Antikörper wird verwendet zum Nachweis von EGFR in Proben von Human und Maus. Er wurde validiert für IF.

Kurzübersicht für EGFR Antikörper (pTyr869) (FITC) (ABIN492341)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EGFR Antikörper ist konjugiert mit FITC

Applikation: Immunofluorescence (IF)

Klon: 12A3

Produktdetails anti-EGFR Antikörper (FITC)

Bindungsspezifität: pTyr869

Spezifität: Clone 12A3 specifically recognizes EGFR phosphorylated at Tyrosine 869 and detects EGFR activation after interaction with src kinases. Antibody AM00031FC-N does not crossreact with the highly homologues pTyr877 of activated erbB2.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Produktmerkmale: Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

Aufreinigung: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Immunogen: Phosphopeptide conjugated to KLHAA Sequence: E K E pY H A ERemarks: Epitope: Phosphotyrosine 869

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry: Use at 1-10 μg/mLUse ABIN126761 or ABIN126759 for Immunoblotting and Immunoprecipitationapplications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide / PEG and Sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-80 °C

Informationen zur Lagerung: Aiquote and freeze in liquid nitrogen. Antibody can be stored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. ThawedAliquots may be stored at 4 °C up to 3 months.

Haltbarkeit: 12 months

Details zu EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung: EGFR / ERBB1

Hintergrund: EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

Gen-ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine