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Nemo-Like Kinase Antikörper

This anti-Nemo-Like Kinase antibody (ABIN4912401) is a Mouse Monoclonal antibody detecting Nemo-Like Kinase in WB. Suitable for Human.
Produktnummer ABIN4912401

Kurzübersicht für Nemo-Like Kinase Antikörper (ABIN4912401)

Target

Alle Nemo-Like Kinase (NLK) Antikörper anzeigen
Nemo-Like Kinase (NLK)

Reaktivität

  • 73
  • 35
  • 12
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 75
  • 3
Maus

Klonalität

  • 74
  • 4
Monoklonal

Konjugat

  • 28
  • 6
  • 5
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Nemo-Like Kinase Antikörper ist unkonjugiert

Applikation

  • 70
  • 31
  • 26
  • 26
  • 19
  • 12
  • 11
  • 10
  • 10
  • 6
  • 1
  • 1
Western Blotting (WB)

Klon

6C1
  • Kreuzreaktivität

    Human

    Aufreinigung

    Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

    Immunogen

    Purified His-tagged NLK protein was used to produced this monoclonal antibody.

    Isotyp

    IgG2a
  • Applikationshinweise

    WB 1:300-5000

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.5 μg/μL

    Buffer

    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

    Konservierungsmittel

    ProClin

    Vorsichtsmaßnahmen

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

    Haltbarkeit

    12 months
  • Target

    Nemo-Like Kinase (NLK)

    Andere Bezeichnung

    NLK

    Hintergrund

    Synonyms: Serine/threonine-protein kinase NLK, Nemo-like kinase, Protein LAK1, NLK, LAK1

    Background: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members. Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed:25512613).

    Gen-ID

    51701

    UniProt

    Q9UBE8

    Pathways

    Ubiquitin Proteasome Pathway
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